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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
15/09/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. |
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Afiliação: |
ISIS VIANA MENDES, Universidade de Brasília; MARIANA BOTELHO GARCIA, Universidade Católica de Brasília; ANA CAROLINA ARAUJO BITENCOURT, Universidade de Brasília; RENATA HENRIQUE SANTANA, Instituto Federal de Brasília; PHILIPPE DE CASTRO LINS, Universidade de Brasília; RAFAELLA SILVEIRA, Universidade de Brasília; BLAKE A. SIMMONS, Joint BioEnergy Institute; JOHN M. GLADDEN, Joint BioEnergy Institute; RICARDO HENRIQUE KRUGER, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Bacterial diversity dynamics in microbial consortia selected for lignin utilization. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 9, e0255083, 2021. |
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Descrição Física: |
PDF: il. color. |
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DOI: |
https://doi.org/10.1371/journal.pone.0255083 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications. MenosAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lign... Mostrar Tudo |
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Thesagro: |
Biotecnologia; Carbono; Composto Fenólico; Lignina. |
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Thesaurus Nal: |
Application technology; Biotechnology; Carbon; Degradation; Energy conversion; Lignin; Phenolic compounds. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226045/1/Bacterial-diversity-dynamics-in-microbial-2021.pdf
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Marc: |
LEADER 03127naa a2200385 a 4500
001 2134421
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0255083$2DOI
100 1 $aMENDES, I. V.
245 $aBacterial diversity dynamics in microbial consortia selected for lignin utilization.$h[electronic resource]
260 $c2021
300 $cPDF: il. color.
520 $aAbstract:Lignin is nature?s largest source of phenolic compounds. Its recalcitrance to enzymatic conversion is still a limiting step to increase the value of lignin. Although bacteria are able to degrade lignin in nature, most studies have focused on lignin degradation by fungi. To understand which bacteria are able to use lignin as the sole carbon source, natural selection over time was used to obtain enriched microbial consortia over a 12-week period. The source of microorganisms to establish these microbial consortia were commercial and backyard compost soils. Cultivation occurred at two different temperatures, 30°C and 37°C, in defined culture media containing either Kraft lignin or alkaline-extracted lignin as carbon source. iTag DNA sequencing of bacterial 16S rDNA gene was performed for each of the consortia at six timepoints (passages). The initial bacterial richness and diversity of backyard compost soil consortia was greater than that of commercial soil consortia, and both parameters decreased after the enrichment protocol, corroborating that selection was occurring. Bacterial consortia composition tended to stabilize from the fourth passage on. After the enrichment protocol, Firmicutes phylum bacteria were predominant when lignin extracted by alkaline method was used as a carbon source, whereas Proteobacteria were predominant when Kraft lignin was used. Bray-Curtis dissimilarity calculations at genus level, visualized using NMDS plots, showed that the type of lignin used as a carbon source contributed more to differentiate the bacterial consortia than the variable temperature. The main known bacterial genera selected to use lignin as a carbon source were Altererythrobacter, Aminobacter, Bacillus, Burkholderia, Lysinibacillus, Microvirga, Mycobacterium, Ochrobactrum, Paenibacillus, Pseudomonas, Pseudoxanthomonas, Rhizobiales and Sphingobium. These selected bacterial genera can be of particular interest for studying lignin degradation and utilization, as well as for lignin-related biotechnology applications.
650 $aApplication technology
650 $aBiotechnology
650 $aCarbon
650 $aDegradation
650 $aEnergy conversion
650 $aLignin
650 $aPhenolic compounds
650 $aBiotecnologia
650 $aCarbono
650 $aComposto Fenólico
650 $aLignina
700 1 $aGARCIA, M. B.
700 1 $aBITENCOURT, A. C. A.
700 1 $aSANTANA, R. H.
700 1 $aLINS, P. de C.
700 1 $aSILVEIRA, R.
700 1 $aSIMMONS, B. A.
700 1 $aGLADDEN, J. M.
700 1 $aKRUGER, R. H.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 9, e0255083, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 9 | |
| 2. |  | MENDES, I. V.; GARCIA, M. B.; SANTANA, R. H.; GLADDEN, J.; KRÜGER, R. H.; QUIRINO, B. F. Análise funcional e da diversidade de um consórcio microbiano capaz de degradar lignina. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 36.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 3. | | COLOMBO, G. S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R.; QUIRINO, B. F. Functional expression of a new xylose isomerase in Saccharomyces Cerevisiae. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió, Anais... Maceió: SBM, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | GARCIA, M. B.; MENDES, I. V.; SANTANA, R. H.; KRUGER, R. H.; GLADDEN, J.; QUIRINO, B. F. Diversidade microbiana e degradação de lignina em cultura enriquecida. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 42.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | COLOMBO, G. de S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Análise filogenética e expressão heteróloga de uma xilose isomerase bacteriana em Saccharomyces cerevisiae. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 101-107 il.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | COLOMBO, G. de S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Expressão funcional de uma nova xilose isomerase em Saccharomyces cerevisiae. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 50.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | COLOMBO, G. de S.; MENDES, I. V.; SOUTO, B. de M.; PARACHIN, N.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Descoberta de novos genes de xilose isomerase em rúmen de cabras brasileiras, In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 16-21.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. Bacterial diversity dynamics in microbial consortia selected for lignin utilization. PLoS ONE, v. 16, n. 9, e0255083, 2021. PDF: il. color.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | COLOMBO, G. DE S.; MENDES, I. V.; SOUTO, B. de M.; BARRETO, C. C.; SERRA, L. A.; NORONA, E. F.; PARACHIN, N. S.; ALMEIDA, J. R. M. de; QUIRINO, B. F. Identification and functional expression of a new xylose isomerase from the goat rumen microbiome in Saccharomyces cerevisiae. Letters in Applied Microbiology, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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